2024 Strep tactin-hrp

Strep tactin-hrp

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August undefined, 2024

WebStrep-Tactin ® HRP conjugate Strep-Tactin ® conjugate for detection of Strep-tag ® II and Twin-Strep-tag ® fusion proteins Read more €127.00* Strep-Tactin ® XT DY-488 conjugate Strep-Tactin ® XT conjugate with fluorescent label to detect Strep-tag ® II and Twin-Strep-tag ® fusion proteins Read more From €188.00* Strep-Tactin ® XT DY-549 conjugate

Hrp Conjugated Strep Tactin IBA Lifesciences Bioz

WebThe streptavidin variant Strep-Tactin is available as conjugate with horseradish peroxidase (HRP) for fast and directed detection of N-terminally, C-terminally, or internally tagged Strep-tagII or Twin-Strep-tag fusion proteins transferred to a blotting membrane or … WebThe affinity system based on the artificial peptide ligand Strep-tag® II and engineered tetrameric streptavidin, known as Strep-Tactin®, offers attractive applications for the study of... initialization\\u0027s nh

利用磁场检测样品中的靶分子 - CN101438163A - 专利顾如

WebWestern Blot with Strep-Tactin® or StrepMAB conjugates - Protocol 3 3.3.5 Directly add 2.5 µl Strep-Tactin®HRP (1:4000). Incubate 60 min at room temperature, gently shaking 3.3.6 … WebUse StrepTactin-Horseradish Peroxidase (HRP) Conjugate for chemiluminescent or colorimetric detection of Precision Plus Protein™ Unstained Protein Standards or Precision Plus Protein™ WesternC™ Protein Standards on western blots. Packaging Options. Number of Applications (Volume) Catalog Number: 150 (300 μl) WebApr 11, 2024 · Therefore, we used the Strep-tag/Strep-Tactin system in this study to isolate proteins bound to AFP. ... reacted with the anti-LAMP-1 antibody and was washed 3 times with TBST buffer and then reacted with a 5000-fold diluted HRP-conjugated anti-rabbit IgG antibody (#W4011, Promega, Alexandria, NSW, Australia). After washing three times with ... initialization\u0027s nx

Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for

Protocol Protein purification with Strep-Tactin resins - IBA …

WebJun 14, 2007 · The Strep -tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity toward streptavidin and can be fused to recombinant proteins in... WebApr 28, 2024 · In this ELISA, each E protein with an OSF tag was captured by Strep-Tactin coated on a plate, and the antibody-E protein complex was detected using Protein A/G (Fig. 2A). mmed afrocentricWebStrep-tag® and Strep-Tactin® Purification and Detection Reagents; DNA Purification; Streptavidin; Immunoprecipiation Using Selector Resins; ... Strep-Tactin HRP Conjugate: 2-1502-001: 0.5 ml: Strep-Tactin® coated microplates: 2-1501-001: 10x96 Well Plate: Strep-Tactin® coated microplates: 2-1501-001: mmed ophthalmology

"Web移液器反复吹吸或短时漩涡混合使磁珠充分重悬； (6) 用户可根据实际需要保留经磁性分离移去的上清液，进行取样检测，以便分析纯化过程和优化蛋白纯化流程； (7) 本产品可以重复使用，重复使用时，建议纯化同种蛋白，纯化不同种类的蛋白时，建议使用新的磁珠，以防交叉污染； (8) 本产品需 " - Strep tactin-hrp

Strep tactin-hrp

Development of a highly specific serodiagnostic ELISA for West …

WebThermo Scientific Pierce High Sensitivity Streptavidin-HRP, 0.5 mL is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability. This specially-manufactured variety of HRP-conjugated streptavidin protein meets the demands of today's scientists for ... WebStrep-Tactin®XT DY-649 Product Details Catalog Number: 2-1568-050 Type: Protein Storage: Undiluted: 2 - 8 °C, Diluted: -25 to -15 °C Shipping: Room Temperature Downloads: Datasheet (pdf) Downloads: MSDS (pdf) Product Sizes For the detection of Strep -tag® II and Twin-Strep-tag in Immunofluorescence, FACS and more.

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WebApplication. Strep-Tag II Antibody HRP Conjugate produces a strong signal at a 1:4000 dilution for dot blots and western blots. Packaging. 75 μL in Plastic ampoule. Warning. … WebMar 30, 2016 · For detection of the constructs, Strep-Tactin®-HRP conjugate was used (IBA, Göttingen, Germany, Cat. no.: 2-1502-001). Samples were run in triplicates on a 10% SDS-PAGE gel and afterwards blotted for 1 h onto a nitrocellulose membrane. The membrane was blocked with 1 x PBS, 0.5% Tween 20 supplemented with 3% BSA for 1 h. ...

Web本申请涉及用于检测可疑含有靶物的样品中的靶物的方法。该方法包括将样品和附着于磁性颗粒上的第一结合分子与附着于固体支持物上的第二结合分子接触。所述第一结合分子能够结合第二结合分子，靶物能够干扰这种结合。施加磁力使得磁性颗粒与固体支持物紧密接近。 WebThe streptavidin variant Strep-Tactin® as conjugate with horseradish peroxidase (HRP) for fast and directed detection of N-terminally, C-terminally, or internally tagged Strep-tag®II …

WebStrep-Tactin® HRP and therefore produces high background. 2. Wash 3 times with 20 ml PBS-Tween buffer (each step: 5 minutes, room temperature, gentle shaking). 3. After the last washing step, add 10 ml PBS-Tween buffer to the membrane. 4. Optional: Before detection of Strep-tag® proteins (step 5) add 10 µl Web同时EGFP的C端含有编码8×His标签的序列和编码短肽亲和标签Strep-tag II的序列，因此可以非常便捷地使用镍柱或Strep-Tactin系统进行分离纯化。同时在目的蛋白和EGFP之间含有PreScission酶切位点，便于后续酶切去除标签。和EGFP融合表达，便于观察表达水平。

WebIt provides a Strep-Tactin®XT 4Flow® column, Buffer W, Buffer BXT, and Buffer XT-R for purification as well as Strep-Tactin® HRP for subsequent western blot detection. Strep-Tactin®XT 4Flow® columns contain a 4% agarose coupled with the streptavidin variant Strep-Tactin®XT. Due to the broad pH compatibility, excellent pressure stability ...

WebStrep-Tactin Superflow Plus （2 ml） 1790.00 : Qiagen: 30004: Strep-Tactin Superflow Plus （10 ml） 7050.00 : Qiagen: 30210: Ni-NTA Agarose （25 ml） 3070.00 : Qiagen: ... RGS·His HRP Conjugate Kit : 4050.00 : Qiagen: 34460: Penta·His HRP Conjugate Kit : 4590.00 : Qiagen: 34530: Ni-NTA HRP Conjugate : 3810.00 : Qiagen: 34610: RGS·His ... initialization\u0027s nkWebOverview. The Strep•Tag II Antibody HRP Conjugate* is a peroxidase-conjugated Strep•Tag II Monoclonal Antibody* that is useful as a single detection reagent thus eliminating cross … mmed neo exchangeWebStrepTactin HRP-Conjugate and AP-Conjugate Instruction Manual Online Resources Protein Electrophoresis Western Blotting Specifications Volume, µl 300 Total number of … m - mediocre string problemWebIBA Lifesciences strep tactin conjugated to horseradish peroxidase 1 Price from $9.99 to $1999.99 strep tactin conjugated to horseradish peroxidase - by Bioz Stars , 2024-03 94 / 100 stars Images strep tactin conjugated to horseradish peroxidase ( IBA Lifesciences ) IBA Lifesciences is a verified supplier IBA Lifesciences manufactures this product m medicationsWebStrep-tag® and Strep-Tactin® Purification and Detection Reagents Human Cells & Tissue Fetal Bovine Serum IBA Lifesciences Products Products Products Overview Antibodies/Markers Antibodies/Markers Overview Primary Antibodies Secondary Antibodies Antibody Supporting Products Human Cells & Tissue Human Cells & Tissue Overview initialization\u0027s nmWebMar 22, 2024 · Description. The streptavidin variant Strep-Tactin™ is available as conjugate with horseradish peroxidase (HRP) or with alkaline phosphatase (AP) for fast and directed detection of N-terminally, C-terminally, or internally tagged Strep-tag™II or Twin-Strep-tag™ fusion proteins transferred to a blotting membrane or captured on a microplate. mmed public healthWebThe small Strep-tag II (WSHPQFEK) can be used to purify active, native conformation proteins to high purity on Strep-Tactin resins.In addition, it can be used in combination with the His tag in a standardized two-step purification procedure. Strep-Tactin is available as Superflow chromatography resin for larger scale purification (up to 9 mg/ml resin). mme divin-boyer