WebRab7 Pull-Down Activation Assay KitCat. # 82501IntroductionA. BackgroundSmall GTPases are a super-family of cellular signaling regulators. The small Ras-like GTPase Rab7 is an … WebProduct overview. Rap1 Activation Assay Kit (ab212011) uses RalGDS RBD Agarose beads to selectively isolate and pull-down the active form of Rap from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rap is detected by western blot analysis using an anti-Rap1 polyclonal antibody, which reacts with the human, mouse and ...
Development of a bioassay to detect T-cell-activating ... - Nature
WebA SUMOylation-dependent switch of RAB7 governs intracellular life and pathogenesis of Salmonella Typhimurium. View PDF In Journal of Cell Science on 11 January 2024 by … WebA published method by Taylor and co-workers uses plasmid-containing Escherichia coli cells to produce a glutathione-S-transferase/raf-1 ras binding domain (GST-RBD) fusion protein attached to glutathione beads to isolate activated ras protein. We systematically optimized the method before use on lung tissues. burton road post office
Rab5 Pull-Down Activation Assay Kit - GTPase and Oncogene
WebSep 14, 2024 · TBC1D15, a member of the TBC (Tre2/Bub2/Cdc16)-domain-containing protein family, is the GTPase-activating protein (GAP) for RAB7. TBC1D15 regulates RAB7 from an active GTP-bound state into an inactive GDP-bound state upon GTP hydrolysis. TBC1D15 is recruited to mitochondria courtesy of binding with Fis1, an outer … WebNewEast Biosciences rab7 activation assay kit Rab7 Activation Assay Kit, supplied by NewEast Biosciences, used in various techniques. Bioz Stars score: 91/100, based on 4 … WebNon-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody. Rac1 Activation Assay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. MSDS. burton road post office lincoln