WebBackground: Phytyl residues are the common side chains of chlorophyll (Chl) and tocopherols. Geranylgeranyl reductase (GGR), which is encoded by CHLP gene, is responsible for phytyl biosynthesis. The light-harvesting like protein LIL3 was suggested to be required for stability of GGR and protochlorophyllide oxidoreductase in Arabidopsis.
Functional Analysis of Light-harvesting-like Protein 3 …
WebJan 12, 2011 · The crystal structure of geranylgeranyl reductase (GGR) from Sulfolobus acidocaldarius was determined in order to elucidate the molecular mechanism of the catalytic reaction. The enzyme is a flavoprotein and is involved in saturation of the double bonds on the isoprenoid moiety of archaeal membranes. The structure determined in this … WebOct 29, 2024 · Background Phytyl residues are the common side chains of chlorophyll (Chl) and tocopherols. Geranylgeranyl reductase (GGR), which is encoded by CHLP gene, … how to add image in css body
A rice geranylgeranyl reductase is essential for chloroplast ...
WebJun 19, 2014 · Structure-guided modification of geranylgeranyl reductase (GGR), reported by Kung et al., enhanced the hydrogenation rate of the universal isoprenoid intermediate GGPP, an unnatural GGR substrate. This study suggests that GGR variants may be used to tailor the products in engineered isoprenoid-producing microorganisms. WebDec 28, 2024 · Geranylgeranyl reductase (GGR) is a flavin-containing redox enzyme that hydrogenates a variety of unactivated polyprenyl substrates, which are further processed mostly for lipid biosynthesis in archaea or chlorophyll biosynthesis in plants. To date, only a few GGR genes have been confirmed to reduce polyprenyl substrates in vitro or in vivo. The Interpro database (EMBL-EBI) predicts over 8000 proteins present within the GGR family (InterPro code: IPR011777), with many homologous genes containing sequence identities as low as 20–30%. After sequence alignment, a phylogeny tree includes 1787 sequences of predicted GGR from the InterPro database. A … See more The 12 soluble proteins successfully isolated were tested for reductase activity on GGOH and FOH, and products obtained after enzymatic incubation were analyzed by GC–MS. … See more The 12 soluble GGRs successfully purified were tested for reductase activity on FPP and GGPP, and products were detected by LC–MS–TOF. Both farnesyl pyrophosphate (FPP, … See more Several synthetic approaches are currently being explored to perform selective hydrogenation on a few substrates [34,35,36]. Biological systems such as enoyl-CoA reductase … See more methodist path lab des moines iowa