WebThus for the Renilla-firefly assay, we used a suboptimal red-shifted 670 +/- 10nm bandpass filter for detection of red firefly, which minimizes interference from green Renilla. As a result, interference was only observed when the output from green Renilla was significantly greater than that from red firefly (Figure 4). To separate spectral data ... WebFirefly/Renilla: Insertion of 3' UTR sequences downstream of Renilla luciferase. Firefly luciferase expression under the control of a HSV TK promoter for normalization: Ting Ni: 174050: pGWB-nLUC: Firefly: Creation of N-terminal Firefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use with 174051
Firefly/Renilla Dual Luciferase Assay SCT152 - EMD Millipore
WebRaw RLUs for firefly and co-reporter (NanoLuc® or Renilla) luciferase when co-reporter DNA amount was titrated against a constant level of firefly luciferase (Fluc). Firefly luciferase vector was maintained at 500ng, while co-reporter (NanoLuc® or Renilla) vector varied from 500pg–50ng. N = 8 for each condition. WebFor Firefly Luciferase, we offer D-Luciferin in a number of formats, including sodium and potassium salts, free acid, and caged. For Renilla Luciferase, we offer many different Coelenterazine analogs, including water-soluble Aquaphile™ Coelenterazines and a Coelenterazine Sampler. Also see our convenient, low-cost Luciferase Reporter Assay … harmony baptist church bastrop la
Dual Luciferase (Firefly-Renilla) Assay System - BPS …
WebEndoFectin™ Max transfection reagent (3 mL) $589. EFL1001-01. EndoFectin™ Lenti transfection reagent (1 mL) $245. Download. EFL1001-02. EndoFectin™ Lenti transfection reagent (3 mL) $489. WebMay 18, 2016 · Traditionally, these systems are based on Firefly and Renilla luciferases; however, the applicability of these enzymes is limited by their size, stability, and luminescence efficiency. NanoLuc (NLuc), a novel bioluminescence platform, offers several advantages over established systems, including enhanced stability, smaller size, and … WebGo-Go™ Fast DNA Gel Running Buffer is a novel low ionic strength running buffer that allows you to run DNA agarose gels at higher voltage to get results up to 3X faster than with TAE or TBE buffer, streamlining routine DNA gel analysis. Go-Go™ Fast buffer also gives crisper band resolution compared to conventional electrophoresis buffers. chaos walking movie plot